RNAlater

Summary

Protocol for the preparation of RNAlater solution

Materials

1. 0.5 M EDTA disodium
2. dihydrate (18.61 g/100 ml,pH to 8.0 with NaOH while stirring);
3. 1 M Sodium Citrate trisodium salt, dihydrate (29.4 g/100 ml, stir to dissolve);
4. Ammonium Sulfate, powdered;
5. Sterile water.

Procedure

1. In a beaker, combine 40 ml 0.5 M EDTA, 25 ml 1 M Sodium Citrate, 700 gm Ammonium Sulfate and 935 ml of sterile distilled water, stir on a hot plate stirrer on low heat until the Ammonium Sulfate is completely dissolved.
2. Allow to cool, adjust the pH of the solution to pH5.2 using 1 M H₂SO₄.
3. Transfer to a screw top bottle and store either at room temperature or refrigerated.
4. Final concentrations:

   25 mM Sodium Citrate
   10 mM EDTA
   70 g ammonium sulfate/100 ml solution
   pH 5.2

References

1. US patent 6204375

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Comments

• Qurat-ul-Ain Amir Monday, 11 December 2023 - 14:08 UTC

  Can this RNA later can be used for whole blood?