50x TAE buffer (50x Tris-acetate-EDTA)

Summary

TAE buffer is typically used for agarose DNA electrophoresis.

Materials

To prepare 1L of 50x solution, you need:

• 242.3 g Tris
• 57.2 mL glacial acetic acid
• 100 mL 0.5M EDTA (pH 8.0)

Procedure

1. Dissolve Tris in about 800 mL of deionized water.
2. Add acetic acid and EDTA.
3. Add deionized water to 1L.
4. Store at room temperature.

Dilute stock solution 50:1 to make a 1x working solution.

1x buffer will contain 40 mM Tris, 20 mM acetic acid and 1 mM EDTA.

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Comments

• 

  Alen Piljić Saturday, 08 March 2014 - 16:35 UTC

  That is true, 1 part buffer, 49 parts water. Dilution is then 1 part in 50 parts total volume, I think it is written correct.

• Arup Kumar Mukherjee Saturday, 08 March 2014 - 14:22 UTC

  Dear Sir,
  From the 50X stock you have to take 1 ml and add 49 ml of de-ionised water to get 1X TAE so, 50:1 is not correct 49 parts water:1 part 50X TAE hould be the correct one.