Authors
Philipp Jungebluth, Bernhard Holzgraefe, Mei Ling Lim, Adil Doganay Duru, Vanessa Lundin, Nina Heldring, Oscar P B Wiklander, Joel Z Nordin, Michael Chrobok, Christoph Roderburg, Sebastian Sjöqvist, Björn Anderstam, Antonio Beltrán Rodríguez, Johannes C Haag, Ylva Gustafsson, Katharina G Roddewig, Petra Jones, Matthew J A Wood, Tom Luedde, Ana I Teixeira, Ola Hermanson, Ola Winqvist, Håkan Kalzén, Samir El Andaloussi, Evren Alici, Paolo Macchiarini
Published in
Respiration; international review of thoracic diseases. Volume 90. Issue 6. Pages 481-92. Epub Nov 28, 2015.
Abstract
Acute respiratory distress syndrome (ARDS) is a devastating disorder. Despite enormous efforts in clinical research, effective treatment options are lacking, and mortality rates remain unacceptably high.A male patient with severe ARDS showed no clinical improvement with conventional therapies. Hence, an emergent experimental intervention was performed.
We performed intratracheal administration of autologous peripheral blood-derived mononuclear cells (PBMCs) and erythropoietin (EPO).
We found that after 2 days of initial PBMC/EPO application, lung function improved and extracorporeal membrane oxygenation (ECMO) support was reduced. Bronchoscopy and serum inflammatory markers revealed reduced inflammation. Additionally, serum concentration of miR-449a, b, c and miR-34a, a transient upregulation of E-cadherin and associated chromatin marks in PBMCs indicated airway epithelial differentiation. Extracellular vesicles from PBMCs demonstrated anti-inflammatory capacity in a TNF-α-mediated nuclear factor-x03BA;B in vitro assay. Despite improving respiratory function, the patient died of multisystem organ failure on day 38 of ECMO treatment.
This case report provides initial encouraging evidence to use locally instilled PBMC/EPO for treatment of severe refractory ARDS. The observed clinical improvement may partially be due to the anti-inflammatory effects of PBMC/EPO to promote tissue regeneration. Further studies are needed for more in-depth understanding of the underlying mechanisms of in vivo regeneration.
PMID:
26613253
Bibliographic data and abstract were imported from PubMed on 07 Jun 2018.
Read full publication at:
Please sign in
to see all details.
Stats
- Recommendations 0 0% positive of 1 vote(s)
- Views 436
- Comments 1
Recommended
Post a comment
Comments
-
Alen Piljić Tuesday, 12 June 2018 - 13:34 UTC
Retraction of this paper was requested by Ole Petter Ottersen, the principal of Karolinska Institutet, but retraction was rejected by the journal. Regardless of the journal decision, this paper should be considered retracted.