Summary
TBE buffer is typically used for agarose DNA electrophoresis.
Materials
To prepare 1L of 5x solution, you need:
- 54 g Tris base
- 27.5 g boric acid
- 20 mL 0.5M EDTA (pH 8.0)
Procedure
- Dissolve Tris in about 800 mL of deionized water.
- Add boric acid and EDTA.
- Add deionized water to 1L.
- Store at room temperature.
Dilute stock solution 5:1 to make a 1x working solution.
0.5x solution can also be used.
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