Materials
Makes 15 ml of 5x Loading Dye:
- 3ml of 20% SDS
- 3.75 mL 1M Tris - pH 6.8
- 9 mg bromphenol blue
- 1.16 mg DTT (Alternatively add 2.4ml B-mercaptoethanol)
- Add water to a final volume of 10.5ml
- Mix the above reagents well
- Add 4.5 mL glycerol
- Mix well again
- Store at 4 degrees C
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