Authors
Vibor Laketa
Materials
Rapamycin (LC Laboratories, Cat.# R-5000)
there is a huge variation (up to 500 fold!!) in rapamycin price depending from which company you order it (for example 50mg of rapamycing will cost you 53$ in LC Laboratories and Sigma-Aldrich charges 502$ for 1mg of rapamycin even with lower purity (prices from March, 2013.)). Rapamycin from LC Laboratories is one of the cheapest (if not the cheapeast) and works excellent in my experiments (rapamycin induced protein dimerisation)
Procedure
m(rapamycin)=50mg
Mr(rapamycin)=914.17 g/mol
dissolve in 2.7ml of DMSO to obtain 20mM stock concentration. Aliquote and store at -20°C.
I use 200nM final concentraion on cells to achieve FRB-FKBP dimerisation.
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