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Rafał Krela

Rafał Krela

Postdoc at Ceske Budejovice

  • Contribution score: 70
  • Publication average: 8.8 (6)
  • Profile views: 710

About

Positions

Postdoc Aug 2021 -

Ceske Budejovice

Evolution of PRC2 in green algae

Researcher Feb 2021 - Jul 2021

Adam Mickiewicz University

Development and verification of the effectiveness of the new COVID-19 immunodiagnostic tool (R&D Grant)

Principal Investigator Feb 2020 - Feb 2023

Adam Mickiewicz University

Analysis of Acanthamoeba castellanii extracellular nucleases function (NCN Preludium Grant)

Researcher Mar 2017 - Mar 2019

Poland

flora robotica | Societies of Symbiotic Robot-Plant Bio-Hybrids as Social Architectural Artifacts (Horizon 2020)

Co-Investigator Dec 2015 - Jul 2018

Poland

Characterization of the mechanism responsible for DNA degradation in different types of plant programmed cell deaths. (NCN Grant)

PhD Student / Researcher Oct 2014 - Jan 2021

Adam Mickiewicz University

Functional analysis of nucleases degrading nucleic acids during programmed cell death in plant cells.

Education

Adam Mickiewicz University in Poznań 2014 - 2021

Field of study: Biology
Degree: PhD

Molecular and functional characterization of nucleases involved in plant programmed cell death.

Adam Mickiewicz University 2012 - 2014

Field of study: Biotechnology
Degree: Master of Science

Catalytic properties of TBN1 nuclease.

Adam Mickiewicz University 2009 - 2012

Field of study: Biotechnology
Degree: Bachelore of Science

Types of programmed cell death.

Skills

 Western Blot, Zymography, Enzyme Assays, SDS Page, Native Page
 Molecular Cloning (restriction enzymes, D-Topo etc.): plasmid construction, protein tag
design, site-direct mutagenesis
 Digitial PCR (EMBO Workshop, Heidelberg 2018)
 Fluorescence and confocal microscopy
 Plant transient and stable transformation (protoplasts, agroinfiltration, floral dip etc.)
 Plant cell cultures stable transformation
 Human cell cultures and amoebas cultures transfection
 GUS reporter gene promoter analysis
 Protein expression and isolation

Professional interests

• Programmed cell death in plants and animals.
• Biology and pathogenicity of protozoa.
• Proteins structure
• Abiotic and biotic stress response in plants

Fellowships

special KNOW scholarship for PhD students 2015 - 2018

special KNOW scholarship for PhD students 2014 - 2015

Grants

Faculty Dean’s Research Grant: : Functional and catalytic characterization of Acanthameoba castelanii DNases II and S1/P1 nucleases. 2016 - 2017

Languages

English, Polish, German

CV

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New opportunities

Open to new opportunities: Yes

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